Improved Oxygen Supply to Multicellular Spheroids Using A Gas-permeable Plate and Embedded Hydrogel Beads
نویسندگان
چکیده
منابع مشابه
Proliferative and clonogenic heterogeneity of cells from EMT6/Ro multicellular spheroids induced by the glucose and oxygen supply.
The culture of EMT6/Ro multicellular tumor spheroids in a 20-fold range of glucose concentrations and a 4-fold range of oxygen tensions had profound effects on the cell subpopulations which developed in the spheroids. As the spheroids increased in diameter the rate of accumulation of cells with a G1-phase DNA content was much greater for those spheroids cultured in the lower oxygen and glucose ...
متن کاملRegulation of growth saturation and development of necrosis in EMT6/Ro multicellular spheroids by the glucose and oxygen supply.
To investigate the effects of glucose and oxygen on spheroid growth, EMT6/Ro mouse mammary carcinoma cell spheroids were cultured in suspension in either 0.28 mM (20%) or 0.07 mM (5%) oxygen and 16.5, 5.5, 1.7, and 0.8 mM glucose. The spheroids initially grew at the same exponential rate in all culture conditions, with spheroid volume and cell number doubling times of 20-24 h. The growth rates ...
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conventional processes of food dewatering, such as thermal, have undesirable and destruction effects on vitamins, aromatic compounds and pigments. in addition, they are accompanied with some technological complications and energy consumption. thus, food concentration processes are directed to non-thermal techniques or methods with reduced heat effects. superabsorbents are highly hydrophilic pol...
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Three-dimensional multicellular spheroids (MCSs) have a complex architectural structure, dynamic cell-cell/cell-matrix interactions and bio-mimicking in vivo microenvironment. As a fundamental building block for tissue reconstruction, MCSs have emerged as a powerful tool to narrow down the gap between the in vitro and in vivo model. In this review paper, we discussed the structure and biology o...
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Most organisms grow according to simple laws, which in principle can be derived from energy conservation and scaling arguments, critically dependent on the relation between the metabolic rate B of energy flow and the organism mass m. Although this relation is generally recognized to be of the form B(m) = m, the specific value of the exponent p is the object of an ongoing debate, with many mecha...
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ژورنال
عنوان ژورنال: Cells
سال: 2019
ISSN: 2073-4409
DOI: 10.3390/cells8060525